Semi-quantitative study of calcitonin gene methylation in myelodyspastic syndrome

摘要

Conclusion: the results suggest that hyperme- thylation of CT gene occurs in early stage of leukemic transformation and CTMR might be a useful marker in predicting the transformation of MDS to AML. Objective To evaluate whether hypermethylation Of calcitonin (CT) gene could serve as a transforming signal of myelodysplastic syndrome (MDS) to leukemi- a. Methods: Bone marrow aspirates from 35 MDS Patients, including 25 refractory anemia (RA), 10 re- fractory anemia with excess of blasts (RAEB) or re- fractory anemia with excess of blasts in transfomation (RAEBt) and 7 cases of acute myeloid leukemia (AML) transformed form MDS, were studied on methylation rate in 5' end of CT gene by polymerase chain reaction (PCR) technique using methylation-sen- sitive endonuclease Hpa Ⅱ with external references of undigested DNA and Msp Ⅰ digested DNA and internal reference of 112 bp fragment containing codon 61 of N- ras oncogene.