Microencapsulation of rat islets prolongs xenograft survival in diabetic mice

摘要

Conclusions Encapsulation of pancreatic islets in semipermeable membranes can effectively prolong xenograft survival without immunosuppression in an animal model. Objective to protect the transplanted islets from the host's immune system by means of immunoexclu- sion membranes. Methods Rat islets were isolated from Wistar rat pancreas by ductal collagenase distention, stationary digestion, and finally with the aid of dextran gradient separation. Then the islets were encapsulated in alginate-polylysine-alginate (APA) semipermeable membranes.