Stable clonal expansion of the T-cell receptor Vβ6, Vβ17 and Vβ 19 T cells in a cGVHD case using genescan analysis

摘要

To investigate the distribution and clonality of the T-cell receptor (TCR) Vβ repertorie in chronic graft versus host disease (cGVHD). Methods The complementarity determining region 3 (CDR3) of the TCRβ gene with 24 variable regions Was amplified in peripheral blood mononuclear cells drawn from one cGVHD patient after allogenic bone Marrow transplantation (allo-BMT) 35, 39, 43 or 45 months respectively, using RT-PCR, to observe the Expression of TCR Vβ repertoire T cells. The PCR products were further analyzed by genescan to evaluate Clonality of T cells.