Molecular Cloning and sequencing of a cDNA encoding partial putative molt-inhibiting hormone from penaeus chinensis

摘要

Total RNA was extracted from eyestalks of shrimp Penaeus chinensis, Eyestalk cDNA was obtained from total RNA by reverse transcription. Reverse transcriptase-polymerase chain reaction (RT-PCR) was initiated using eyestalk cDNA and degenerate primers designed from the amino acid se- quence of molt-inhibiting hormone from shrimp Penaeus japonicus. A specific cDNA was obtained and cloned into a T vector for sequencing. The cNDA consisted of 201 base pairs and encoding for a peptide of 67 amino acid residues. The peptide of P. Chinensis had the highest identity with molt-inhibiting hor- mones of P. Japonicus. The cDNA could be a partial gene of molt-inhibiting hormones from P. Chinen- sis. This paper reports for the fist time cDNA encoding for neuropeptide of P. chinensis.