Monitoring wastewater discharge from the oil and gas industry using passive sampling and Danio rerio bioassay as complimentary tools

摘要

Produced water (PW) represents the largest volume waste stream in oil and gas production operations from most offshore platforms. PW is difficult to monitor as releases are rapidly diluted and concentrations can reach trace levels. The use of passive samplers can over come this. Here polyethylene (PE) was calibrated for a diverse range of PW pollutants. Zebrafish were exposed to dilutions of PW and passive sampler extracts in order to investigate the relationship between freely dissolved chemical concentrations and acute toxic effects. The raw PW had an LC50 of 13% (percentage of PW in the standardized zebrafish medium). Observed non-viable deformations to embryos (at 5 hpf) included heart and yolk edema, head, spine and tail deformations. The dose-response relationship of lethal effects showed that if 0.0041 g of PE is exposed to this PW, then extracted, 50% of exposed D. rerio will suffer lethal effects. The sum of tested freely dissolved concentrations that led to 50% lethal effects (mortality and non-viable deformations) was 2.32 x 10(-4) mg/L for PW and 7.92 x 10(-2) mg/L for PE. This implies that exposure to raw PW was more toxic than exposure to PE extracts. This toxicity was attributed both to the presence of contaminants as well as PW salinity. Passive samplers are able to detect very low freely dissolved pollutant concentrations which is important for assessing the spatial dilution of PW releases. Bioassays provide complimentary information as they account for all toxic compounds including those that are not taken up by passive samplers. (C) 2018 Elsevier Ltd. All rights reserved.