Fluorescent Assay Of Dna Hybridization With Label-free Molecular Switch: Reducing Background-signal And Improving Specificity By Using Carbon Nanotubes

摘要

Using classic DNA-intercalating dye and carbon nanotubes, a simple, but efficient, method for fluorescent detection of DNA hybridization has been developed.rnWe have developed a simple, but efficient, method for nucleic acid detection using classic DNA-intercalating dye and single-walled carbon nanotubes (SWNTs). Current fluorescence methods for DNA detection based on a hybridization assay focus on the design of a single-stranded DNA (ssDN A) with a dye-quencher pair. However, the necessity of dye-quencher pair optimization and sophisticated probe design and synthesis limited their application as common biosensing approaches. An alternative strategy uses DNA-intercalating dyes to recognize and signal DNA hybridization. Specifically, dyes show different binding abilities to double-stranded DNA (dsDNA) over ssDNA.