From DNA cross-linking to peptide labeling: on the versatility of the furan-oxidation-conjugation strategy

摘要

Based on the incorporation of commercially available N-Fmoc-furylalanine, a new method for peptide labeling is proposed, relying on the selective oxidative transformation of the furan moiety into a reactive aldehyde and subsequent reductive amination.rnModern chemical biology oriented research frequently relies on the ability to site-selectively modify biomacromolecules such as peptides, carbohydrates and oligonucleotides. Although the so called "click" 1,3-dipolar cycloaddition between alkynes and azides has revolutionized the field of bioconjugation chemistry, it does require the introduction of unnatural functionality in both partners.