An artificial metalloenzyme for olefin metathesis

摘要

A Grubbs-Hoveyda type olefin metathesis catalyst, equipped with an electrophilic bromoacetamide group, was used to modify a cysteine-containing variant of a small heat shock protein from Methanocaldococcus jannaschii. The resulting artificial metalloenzyme was found to be active under acidic conditions in a benchmark ring closing metathesis reaction.In recent years, artificial metalloenzymes have emerged as alternative tools to complement organometallic-, enzymatic-, heterogeneous- and organo-catalysis. Artificial metalloenzymes result from incorporation of a catalytically competent organometallic moiety within a macromolecule (protein or DNA).