Selective fluorescent-free detection of biomolecules on nanobiochips by wavelength dependent-enhanced dark field illumination

摘要

Individual silver nanoparticle-conjugated target protein (cTnI) molecules on gold-nanopattemed chip were selectively detected by wavelength dependent-enhanced dark field illumination. Using specific nanoparticles with unique sizes and materials, the immunotargeted nanoparticle on the chips was detected at the single-molecule level by monitoring changes in the plasmonic resonance based on wavelength dependence.