Pharmacological characterization of β_2-adrenoceptor in PGT-β mouse pineal gland tumour cells

摘要

1. The adrenoceptor in a mouse pineal gland tumour cell line (PGT-β) was identified and characterized using pharmacological and physiological approaches. 2. Adrenaline and noradrenaline, adrenoceptor agonists, stimulated cyclic AMP generation in a concentration-dependent manner, adrenoceptor agonists, stimulated cyclic AMP generation in a concentration-dependent manner, but had no effect on inositol 1, 4, 5-trisphosphate production. Adrenaline was a more potent activator of cyclic AMP generation than noradrenaline, with half Maximal-effective concentrations (EC_50) seen at 175 ± 22 nM and 18 ± 2 μM for adrenaline and Noiradrenaline, respectively.