Preparation of affinity sorbents and isolation of individual chitinases from a crude supernatant produced by Streptomyces kurssanovii by a one-step affinity-chromatographic system

摘要

The preparation of two affinity-chromatography sorbents based on cross-linked chitin are described. Both sorbents retained selectively one of the four extracellular chitinases in the culture supernatant produced by Streptomyces kurssanovii. Chitinases with molecular masses of 42 kDa and 26 kDa were isolated in homogeneous form using one-step affinity-chromatography procedures involving either a fully N-acetylated or a partially N-acetylated cross-linked chitin-type sorbent. Two other chitinases were not selectively bound by the sorbents and, therefore, were not isolated in a homogeneous form. The affinity sorbents were shown to be stable over the period of separation and could be used repeatedly.