Backbone resonance assignments for the cytoplasmic regions of G protein-activated inwardly rectifying potassium channel 1 (GIRK1)

摘要

G protein-activated inwardly rectifying potassium channel (GIRK) plays crucial roles in regulating heart rate and neuronal excitability in eukaryotic cells. A variety of ligands, including heterotrimeric G protein βγ subunits (Gβγ), bind to the cytoplasmic regions of GIRK and modulate its activity. We established the backbone resonance assignments of 2H/13C/15N-labeled cytoplasmic regions of mouse GIRK1, which form a tetramer with a molecular weight of 96 K.