Localition of two domains of a mutant form of Escherichia coli protein L7/L12 that binds the large ribosomal subunit as a single dimer

摘要

Escherichia coli ribosomal protein L7/L12 occurs on the large subunit as two dimmers: one dimmer is extended and comprises the stalk, while the second dimmer is folded and occupies a site on the subunit body. A variant protein, in which all 18 amino acids of the flexible hinge region that links separate N-terminal and C-terminal domains of L7/L12 has been deleted, binds the subunit as a single dimmer and does not generate stalks that are visible in electron micrographs.