Biphasic pattern of heparin-induced oxidative degradation of trypsin in the presence of glucose

摘要

The present work tested the hypothesis that the oxidative effects of heparin on trypsin may be modified by the addition of another reducing substance. Glucose was chosen as reducing sugar and used at a concentration (15 mM) and an incubation time (5 h at 37 deg C) with trypsin which may cause only modest oxidative modifications, thus resembling what happens in vivo in the presence of slight long-lasting hyperglycaemia.