Perfluoroalky1phosphocholines are poor protein-sco1ubilizing surfactants, as tested with neutrophi1 plasma membranes

摘要

We have tested the membrane-protein solubilizing properties of two perfluoroalky1phochoilnes. These compounds belong to a series of fluorinated amphiphiles which are being investigated as potential stabilizing agents for a variety of fluorocarbon-based systems. We are particularly interested in cytochrome b_558 from phagocytes, the redox component of NADPH oxidase. Its heavy subunit is believed To carry binding sites for NADPH and FAD. Nevertheless, when the cytochrome is purified in the presence of classical detergents, it carries No FAD. This could be due to a delipidating, denaturing effect of these detergents(octyl glucoside, Triton, etc).