Analysis on ‘Whole Red’ of patterns Oujiang colour carp (Cyprinus carpio var.color?) by SRAP and SCAR markers

摘要

The sequence-related amplified polymorphism (SRAP) technique was applied to identify the genetic difference between the ‘Whole Red’ (‘WR’) population and the ‘Whole White’ (‘WW’) population. The specific SRAP band, namely SR_2,7173?bp (SR indicates the first two letters of SRAP) from ‘WR’ and ‘WW’, was identified from the amplified bands of 12 primer pairs (PP) screened from 88 SRAP PP. After gel extraction, cloning and sequencing of specific band from the two populations, the sequence was submitted to database of Genome Sequence Survey. blast analysis showed that this SRAP fragment shared high similarity to functional genes of Danio rerio. Four primers (22–26 bases) were designed according to the sequence information. Then, polymerase chain reaction amplification was carried out in the two populations. The experimental results also showed that among four sequence-characterized amplified region (SCAR) markers, only SC-3 (154?bp) developing from SR_2,7173?bp (me2-em7) showed specificity to ‘WR’. Examination with a large sample size showed that SCAR 3 (SC-3) could be positively amplified with 85% frequency in the ‘WR’ population rather than in the ‘WW’ population. The results indicated that the SC-3 marker could be used as a specific molecular marker for population identification, providing an effective, easy and rapid method for discriminating different populations and conducting genetic analysis.