Interactions of Vitamin K3 with Herring-Sperm DNA Using Spectroscopy and Electrochemistry

摘要

By means of ultraviolet–visible (UV-Vis) and fluorescence spectra, thenbinding ratio between vitamin K3 and herring-sperm DNA in anphysiological pH environment (pH ¼ 7.40) was determined as nK3 : nDNAn¼ 2 : 1, and the binding constants of vitamin K3 binding to DNA atndifferent temperatures were determined as Khn298K ¼ 1.28 6 105nLu0002molnu00031nand Khn310K ¼7.19 6 104nLu0002molnu00031n, which were confirmed using the doublenreciprocal method are DrHmhn¼ u00033.57 6 104nJu0002molnu00031n, DrGmhn¼ u00032.92 6n104nJu0002molnu00031n, and DrSmhn¼ 217.67 Ju0002molnu00031nKu00031n. The driving power of thisnprocess was enthalpy. An intercalation binding of the vitamin K3 withnDNA was supported by a competitive experiment using acridine orangen(AO) as a spectral probe. By combination analysis of the Scatchardnmethod and cyclic voltammetry, we suggested that the interaction modenbetween vitamin K3 and herring-sperm DNAwould be a mixed mode. Thenquinonoid, duality fused-ring of vitamin K3 can intercalate into the basenpairs of DNA, and there is an electrostatic binding along withnintercalation binding.