Protective effect of resveratrol on formation of membrane protein carbonyls and lipid peroxidation in erythrocytes subjected to oxidative stress

摘要

Plusieurs des actions biologiques du resve ´ratrol de ´coulent de ses proprie ´te ´s antioxydantes. Dans cette e ´tude, onnsoumet des e ´rythrocytes humains a ` un stress oxydatif in vitro en les incubant en pre ´sence de tert-butylhydroperoxyden(t-BHP), augmentant ainsi significativement le niveau de malonalde ´hyde (MDA) et la concentration de carbonyle dans lanprote ´ine membranaire, et ce, au-dessus des valeurs de re ´fe ´rence. La concentration micromolaire de trans-resve ´ratrol dansnle milieu d’incubation prote `ge les e ´rythrocytes du stress oxydatif suscite ´ par le t-BHP comme le re ´ve `le la diminution dunniveau de MDA et de la concentration de carbonyle dans la prote ´ine membranaire. L’effet du resve ´ratrol de ´pend de sanconcentration et de la dure ´e de sa pre ´sence. Nos observations contribuent a ` ame ´liorer les connaissances concernant lesneffets be ´ne ´fiques du resve ´ratrol.%Many of the biological actions of resveratrol have been attributed to its antioxidant properties. In this work, wensubjected human erythrocytes to in vitro oxidative stress by incubating them with tert-butylhydroperoxide (t-BHP). Thisncaused a significant increase in the malondialdehyde (MDA) level and the protein carbonyl group content above the basalnvalues. The presence of trans-resveratrol at micromolar concentrations in the incubation medium protected the erythrocytesnfrom t-BHP-induced oxidative stress, as evidenced by the decrease in the MDA level and the protein carbonyl group con-ntent. The effect of resveratrol was concentration and time-dependent. Our findings help to explain some of the beneficialneffects of resveratrol.