首页> 外文期刊>Applied Physics Letters >Selective excitation of tryptophan fluorescence decay in proteins using a subnanosecond 295 nm light-emitting diode and time-correlated single-photon counting
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Selective excitation of tryptophan fluorescence decay in proteins using a subnanosecond 295 nm light-emitting diode and time-correlated single-photon counting

机译:使用亚纳秒295 nm亚发光二极管和与时间相关的单光子计数,选择​​性激发蛋白质中色氨酸荧光衰减

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摘要

We demonstrate an AlGaN light-emitting diode (LED) giving pulses of ~600 ps full width half maximum, 0.35 μW average power, 0.6 mW peak power, and ~12 nm bandwidth at 295 nm. This source is ideal for protein intrinsic tryptophan fluorescence decay research without the unwanted excitation of tyrosine and paves the way to lab-on-a-chip protein assays using fluorescence decay times. Fluorescence decay and anisotropy decay measurements of human serum albumin are reported and the usefulness of the 295 nm LED demonstrated in comparisons with a nanosecond flashlamp and LEDs with nominal wavelength emission of 280 nm.
机译:我们演示了一个AlGaN发光二极管(LED),其脉冲全宽度的一半为〜600 ps,平均功率为0.35μW,峰值功率为0.6 mW,在295 nm处的带宽为〜12 nm。该源非常适合蛋白质内在色氨酸的荧光衰减研究,而无需酪氨酸的不必要激发,并为使用荧光衰减时间进行芯片实验室蛋白质测定铺平了道路。报告了人类血清白蛋白的荧光衰减和各向异性衰减测量,并且与纳秒级闪光灯和标称波长为280 nm的LED进行了比较,证明了295 nm LED的有用性。

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