Quantitatively linking collagen alteration and epithelial tumor progression by second harmonic generation microscopy

摘要

Collagen alteration is critical for epithelial tumor initiation and progression. Quantitatively linking collagen alteration and epithelial tumor progression is essential for developing an optical endoscopy to evaluate epithelial tumor progression. In this work, we established a quantitative link between collagen alteration and epithelial tumor progression using second harmonic generation (SHG) microscopy. It was found that SHG microscopy can provide quantitative features to effectively evaluate epithelial tumor progression, and to locate tumor and determine the margin of tumor regions. These results suggest that SHG microscopy has the potential in offering a noninvasive in vivo imaging tool to quantify epithelial tumor progression.