Generating Vesicular Stomatitis Virus Pseudotype Bearing the Severe Acute Respiratory Syndrome Coronavirus Spike Envelope Glycoprotein for Rapid and Safe Neutralization Test or Cell-Entry Assay

摘要

We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Δ G~*SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARS-CoV in trans. The neutralization and infection blocking tests showed that the VSV Δ G~*SG and SARS-CoV reacted similarly to SARS-CoV specific antiserum, suggesting the VSV Δ G~*SG can be a safe replacement of the live SARS-CoV for neutralization test and cell-entry assay.