Interfacing Cell-Based Assays in Environmental Scanning Electron Microscopy Using Dielectrophoresis

摘要

ABSTRACT:Development of the dielectrophoretic (DEP) livencell trapping technology and its interfacing with the environ-nmental scanning electron microscopy (ESEM) is described.nDEP microelectrode arrays were fabricated on glass substratenusing photolithography and lift-off. Chip-based arrays werenapplied for ESEM analysis of DEP-trapped human leukemicncells. This work provides proof-of-concept interfacing ofnthe DEP cell retention and trapping technology with ESEMnto provide a high-resolution analysis of individual nonadherent cells.