In Vitro Fertilization on a Single-Oocyte Positioning System Integrated with Motile Sperm Selection and Early Embryo Development

摘要

ABSTRACT: In vitro fertilization (IVF) technology has beennbroadly applied to solve human infertility in recent years.nHowever, the physical tools for IVF remain unchanged overnseveral decades before microfluidic technology was introducednin this field. Here, we report a novel microdevice that integratesneach step of IVF, including oocyte positioning, spermscreening,nfertilization, medium replacement, and embryo culture. Oocytes can be singly positioned in a 4 u0003 4 array of octacolumn units. Thenfour symmetrical straight channels, crossing at the oocyte positioning region, allowed efficient motile sperm selection and facilitatednrapid medium replacement. The fertilization process and early embryonic development of the individual zygote was traced withnmicroscopic recording and analyzed by in situ fluorescent staining. The murine sperm motility was increased from 60.8 ( 3.4% ton96.1 ( 1.9% through the screening channels. The embryo growth rate and blastocyst formation were similar between the routinenPetri dish group and the microdevice group. The healthy blastocysts developed in the microdevice could be conveniently retrievednthrough a routine pipetting operation and used for further embryo transfer.