Modulating effect of mitomycin or cisplatin on lymphokine-activated killer cell proliferation and antitumor activity to bladder cancer cell lines in vitro

摘要

Conclusion: Immunomodulatory effect of chemotherapeutic Agents on LAK cell proliferation induced by IL-2 In patients with bladder cancer mainly depends On the drug itself. Aim: To study the effect of mitomycin(Mit)or Cisplatin(Cis)on the proliferation of Lymphokine-activated killer(LAK)cells in Patients with transitional cell cancer of bladder And their cytolysis to bladder tumor cells. Methods: LAK cell proliferation was assayed In the presence of Mit or Cis by cell counting. Bladder cancer cell lines BIN-87 and EJ were Cultured as target cells and cytoxicity of LAK Cells was determined by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay.