Identification and expression analysis of genes in response to high-salinity and drought stresses in Brassica napus

摘要

High salinity and drought are the major abiotic stressesnthat adversely affect plant growth and agricultural pro-nductivity. To investigate genes that are involved innresponse to abiotic stresses in Brassica napus, a compre-nhensive survey of genes induced by high-salinity andndrought stresses was done by macroarray analysis. Inntotal, 536 clones were identified to be putative high-sal-ninity- or drought-responsive genes. Among them, 172 andn288 clones are detected to be putative high-salinity- andndrought-inducible genes, whereas 141 and 189 are candi-ndates for high-salinity- and drought-suppressed genes,nrespectively. The functional classification of these genesnare indicated that belonged to gene families encodingnmetabolic enzymes, regulatory factors, components ofnsignal transduction, hormone responses, some abioticnstresses-related proteins, and other processes related tongrowth and development of B. napus. From the up-nregulated candidate genes, some interested genes werenfurther demonstrated to be high-salinity- or/and drought-ninduced expression by real-time quantitative RT-PCRnanalysis. The experimental results also revealed that somengenes may function in abscisic acid-dependent signalingnpathway related to drought or salinity stress. Collectively,nthe data presented in this study will facilitate the under-nstanding of molecular mechanism of B. napus in responsento high-salinity and drought stresses, and also provide usnthe basis of effective genetic engineering strategies fornimproving stress tolerance of B. napus.