The present study focused on fermentative butyric acid production by Clostridium tyrobutyricum from pre-treated and hydrolysed wheat straw (PHWS) based on continuous operation mode and in situ acids extraction by reverse electro enhanced dialysis (REED). Different dilutions of PHWS in a synthetic medium (60–100 % v/v) were tested. It was found that continuous fermentation of PHWS greatly enhanced the sugar consumption rates and butyric acid productivity compared to batch tests, while application of REED enhanced them even further. Specifically, applying combined continuous operation mode and REED system for the fermentation of 70 % PHWS resulted in 19- and 53-fold higher glucose (1.37 g L−1 h−1) and xylose (0.80 g L−1 h−1) consumption rates, respectively, compared to those obtained by batch processing. Fermentation of 100 % PHWS continued unhindered with just urea and K2HPO4 added with butyric acid production rate, yield and selectivity being 1.30 g L−1 h−1, 0.45 g g−1 sugars and 0.88 g g−1 acids, respectively. These results were also confirmed in a 20 L pilot plant bioreactor system.
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机译：本研究的重点是基于连续操作模式并通过逆电增强渗析（REED）原位提取酸，由预处理和水解的小麦秸秆（PHWS）生产的酪丁酸梭状芽孢杆菌发酵性丁酸。测试了PHWS在合成培养基中的不同稀释度（60-100％v / v）。结果发现，与批次试验相比，PHWS的连续发酵极大地提高了糖消耗率和丁酸生产率，而REED的应用甚至进一步提高了糖消耗率和丁酸生产率。具体而言，将连续运行模式和REED系统组合用于70％PHWS的发酵会导致葡萄糖分别高19倍和53倍（1.37 g L -1 h -1 ）和木糖（分别为0.80 g L -1 h -1 ）的消耗率，与通过批处理获得的消耗率相比。仅添加尿素和K2HPO4并添加丁酸，即可继续不受阻碍地发酵100％PHWS，产量和选择性为1.30 g L -1 h -1 ，0.45 g g < sup> -1 糖和0.88 g g -1 酸。在20 L中试植物生物反应器系统中也证实了这些结果。
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