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Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE)

机译:Wistar Han RCC大鼠饲喂两种转基因玉米MON810品种90天的体液和细胞免疫反应(欧盟第七框架计划项目GRACE)

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摘要

The genetically modified maize event MON810 expresses a Bacillus thuringiensis-derived gene, which encodes the insecticidal protein Cry1Ab to control some lepidopteran insect pests such as the European corn borer. It has been claimed that the immune system may be affected following the oral/intragastric administration of the MON810 maize in various different animal species. In the frame of the EU-funded project GRACE, two 90-day feeding trials, the so-called studies D and E, were performed to analyze the humoral and cellular immune responses of male and female Wistar Han RCC rats fed the MON810 maize. A MON810 maize variety of Monsanto was used in the study D and a MON810 maize variety of Pioneer Hi-Bred was used in the study E. The total as well as the maize protein- and Cry1Ab-serum-specific IgG, IgM, IgA and IgE levels, the proliferative activity of the lymphocytes, the phagocytic activity of the granulocytes and monocytes, the respiratory burst of the phagocytes, a phenotypic analysis of spleen, thymus and lymph node cells as well as the in vitro production of cytokines by spleen cells were analyzed. No specific Cry1Ab immune response was observed in MON810 rats, and anti-maize protein antibody responses were similar in MON810 and control rats. Single parameters were sporadically altered in rats fed the MON810 maize when compared to control rats, but these alterations are considered to be of no immunotoxicological significance.Electronic supplementary materialThe online version of this article (10.1007/s00204-018-2230-z) contains supplementary material, which is available to authorized users.
机译:转基因玉米事件MON810表达了苏云金芽孢杆菌衍生基因,该基因编码杀虫蛋白Cry1Ab来控制一些鳞翅目害虫,例如欧洲玉米bore。已经声称,在各种不同动物物种中口服/胃内施用MON810玉米后,免疫系统可能受到影响。在欧盟资助的GRACE项目的框架内,进行了两个90天的喂养试验,即所谓的D和E研究,以分析饲喂MON810玉米的雄性和雌性Wistar Han RCC大鼠的体液和细胞免疫反应。在研究D中使用的是Monsanto的MON810玉米品种,在研究E中使用的是Pioneer Hi-Bred的MON810玉米品种。玉米蛋白和Cry1Ab血清特异性IgG,IgM,IgA和IgE水平,淋巴细胞的增殖活性,粒细胞和单核细胞的吞噬活性,吞噬细胞的呼吸爆发,脾,胸腺和淋巴结细胞的表型分析以及脾细胞在体外产生的细胞因子分析。在MON810大鼠中未观察到特异性Cry1Ab免疫反应,在MON810和对照大鼠中抗玉米蛋白抗体反应相似。与对照大鼠相比,用MON810玉米喂养的大鼠的零星参数有零星变化,但这些变化被认为没有免疫毒理学意义。电子补充材料本文的在线版本(10.1007 / s00204-018-2230-z)包含补充信息资料,可供授权用户使用。

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