An automatic flow-injection method for the determination of malate dehydrogenase activity is proposed. The manifold used includes a selecting valve for closing the circuit along which the reacting plug is continuously circulated, and passed through the flow-cell of a conventional spectrophotometer, inserted into the closed circuit. A multipeak recording is obtained, each peak corresponding to one passage of the plug through the flow-cell. This recording allows the sensitivity to be modified, as required, using different types of measurements (for example, absorbance of the maxima or minima and absorbance differences between maxima or minima or their sums). The activity of the analyte can be determined in the range 0.02-2.00 U/l, with an r.s.d, of less than 0.90% at a sampling rate between 11 and 65 samples/h. The applicability of the method was checked by applying it to human serum samples. Analyte recoveries between 96 and 103% were obtained.
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机译：提出了一种自动流动注射法测定苹果酸脱氢酶活性的方法。所使用的歧管包括用于关闭回路的选择阀，反应塞沿着该选择阀连续地循环，并通过常规分光光度计的流通池，并插入到封闭回路中。获得多峰记录，每个峰对应于塞子通过流通池的一个通道。此记录允许使用不同类型的测量（例如，最大值或最小值的吸光度以及最大值或最小值或它们的总和之间的吸光度差异）根据需要修改灵敏度。分析物的活性可以在0.02-2.00 U / l的范围内确定，r.s.d小于0.90％，采样率为11至65个样本/小时。通过将该方法应用于人血清样本来检查该方法的适用性。获得的分析物回收率为96％至103％。
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