Identification of proteins from the secretory/excretory products (SEPs) of the branchiuran ectoparasite Argulus foliaceus (Linnaeus 1758) reveals unique secreted proteins amongst haematophagous ecdysozoa

摘要

SDS-PAGE of secretions. Secretory/excretory products (SEPs) were collected and proteins separated on 12% SDS-PAGE gels and stained with QC colloidal Coomassie stain ( ) and silver stain ( ) to visualise the protein bands. Numbers on the left indicate the approximate molecular mass (MW; kDa) of the proteins within the most diluted sample. Ten distinct bands (arrows) were observed after staining the gels. Molecular mass (2–250 kDa) marker is shown in the middle between the two gels. Asterisk indicates the lane from the Coomassie stained gel that was selected for GeLC-MS/MS analysis. No bands were seen in the water control