ct-DNA Binding and Antibacterial Activity of Octahedral Titanium (IV) Heteroleptic (Benzoylacetone and Hydroxamic Acids) Complexes

摘要

Five structurally related titanium (IV) heteroleptic complexes, [TiCl2(bzac)(L^1–4)] and [TiCl3(bzac)(HL⁵)]; bzac = benzoylacetonate; L^1–5 = benzohydroximate (L¹), salicylhydroximate (L²), acetohydroximate (L³), hydroxyurea (L⁴), and N-benzoyl-N-phenyl hydroxylamine (L⁵), were used for the assessment of their antibacterial activities against ten pathogenic bacterial strains. The titanium (IV) complexes (>1–>5) demonstrated significant level of antibacterial properties as measured using agar well diffusion method. UV-Vis absorption spectroscopic technique was applied, to get a better insight into the nature of binding between titanium (IV) complexes with calf thymus DNA (ct-DNA). On the basis of the results of UV-Vis absorption spectroscopy, the interaction between ct-DNA and the titanium (IV) complexes is likely to occur through the same mode. Results indicated that titanium (IV) complex can bind to calf thymus DNA (ct-DNA) via an intercalative mode. The intrinsic binding constant (K b) was calculated by absorption spectra by using Benesi-Hildebrand equation. Further, Gibbs free energy was also calculated for all the complexes.