Characterization of blood flow and the effects of exogenous estradiol benzoate on residual follicles formed after ultrasound-guided transvaginal follicle aspiration in cattle

摘要

BackgroundRemoval of the follicular content by ultrasound-guided transvaginal follicle aspiration (TVFA) may fail to induce immediate atresia and loss of function, resulting in the occurrence of residual follicles (RF). The aims of this study were to characterize the blood flow in RF and to determine the effects of the treatment with estradiol benzoate on RF fate. Lactating, cyclic Holstein-Gir crossbred cows were used. In Experiment 1, follicular wave emergence (D0) was synchronized in cows (n = 10) and follicular growth was then monitored by transrectal ultrasonography from D0 to D8, followed by TVFA of the largest follicle present on the ovaries 24 h later. Color Doppler ultrasound imaging was used to examine blood flow on the follicular wall, which was recorded immediately before and every 12 h after TVFA, up to 72 h. In experiment 2, cows (n = 22) were randomly allocated to receive either 2 mL of saline i.m. (Control group, n = 11) or 2 mL estradiol benzoate i.m. (EB group, n = 11) immediately after TVFA. Ovaries were scanned every 12 h to confirm the presence and to measure the diameter of RF. The contents of the RF, if present, were collected 72 h after the first TVFA, using the same aspiration procedures. Follicular fluid from original follicles and RF were stored at -20 °C until hormonal assays.