Catalase-Aminotriazole Method for Measuring Secretion of Hydrogen Peroxide by Microorganisms

摘要

A new method for measuring the secretion of H2O2 has been based upon an H2O2-dependent inhibition of catalase by 3-amino-1,2,4-triazole. The conversion of an H2O2-secretion rate into a catalase inhibition rate amplified a relatively small molar concentration of H2O2 and provided a highly specific and sensitive method for quantitatively measuring H2O2. A major advantage of this approach is that it does not require extensive accumulation of H2O2 in the environment. The method was successfully employed to measure H2O2 secretion by Mycoplasma pneumoniae, which possesses a peroxidase-like activity that limits the accumulation of H2O2 in the environment.