Davis, J. B. (Socony Mobil Oil Co., Inc., Dallas, Tex.), V. F. Coty, and J. P. Stanley. Atmospheric nitrogen fixation by methane-oxidizing bacteria. J. Bacteriol. >88:468–472. 1964.—Methane-oxidizing bacteria capable of fixing atmospheric nitrogen were isolated from garden soil, pond mud, oil field soil, and soil exposed to natural gas, indicating a rather wide prevalence in nature. This may explain the high concentration of organic nitrogen commonly found in soils exposed to gas leakage from pipelines or natural-gas seeps. Added molybdenum was a requirement for growth in a nitrogen-free mineral salts medium. All nitrogen-fixing, methane-oxidizing bacteria isolated were gram-negative, nonsporeforming, usually motile rods. Colonies were light yellow, yellow, or white. The most common isolate, which formed light-yellow colonies, is referred to as Pseudomonas methanitrificans sp. n., and is distinguished from Pseudomonas (Methanomonas) methanica by nitrogen-fixing ability and a preponderance of poly-β-hydroxybutyrate in the cellular lipid fraction.
展开▼
机译：戴维斯（Davis），J。B.（Socony Mobil Oil Co.，Inc.，得克萨斯州达拉斯），V。F. Coty和J. P. Stanley。甲烷氧化细菌在大气中固氮。 J.细菌。 > 88： 468–472。 1964年。从花园土壤，池塘泥浆，油田土壤和暴露于天然气的土壤中分离出能够固定大气中氮的甲烷氧化细菌，这表明自然界中存在相当普遍的细菌。这可以解释通常在暴露于管道或天然气渗漏气体的土壤中发现的高浓度有机氮。添加钼是在无氮无机盐培养基中生长的必要条件。分离出的所有固氮甲烷氧化细菌均为革兰氏阴性，无孢子形成，通常为活动杆。菌落为浅黄色，黄色或白色。形成浅黄色菌落的最常见分离株称为假单胞菌methanitrificans sp。 n。，并且与固氮假单胞菌（Methanomonas）甲烷甲烷菌的区别在于固氮能力和细胞脂质组分中聚-β-羟基丁酸酯的优势。
展开▼
