Dendrobium longicornu Lindl is an epiphytic orchid with significant ornamental values. Here, we report the first complete chloroplast genome of D. longicornu. The complete chloroplast (cp) genome sequence of D. longicornu is 160,024 bp in length and consisted of two inverted repeats (IRs, 25,403 bp), which were separated by a large single copy region (LSC, 88,075 bp) and a small single copy region (SSC, 21,143 bp). The cp genome encoded 142 genes, of which 110 were unique genes (80 protein-coding genes, 26 tRNAs and 4 rRNAs). Phylogenetic analysis showed that D. longicornu clustered together with D. ellipsophy.
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