Combined FGFR and Akt pathway inhibition abrogates growth of FGFR1 overexpressing EGFR-TKI-resistant NSCLC cells

摘要

a Tolerance to erlotinib and afatinib was assessed in the parental HCC827 and the three erlotinib-resistant cell lines, ER10, ER20, and ER30, by crystal violet assay after 72 h incubation. Data are presented as the average of four replicates and shown as mean ± SD. b Selected proteins significantly regulated across the three erlotinib-resistant cell lines compared to the parental HCC827, as assessed by mass spectrometry-based proteomics, were classified according to function. The dotted red line indicates 2-fold regulation. c Western blot analysis of total and phosphorylated levels of selected signaling proteins. The HCC827 cell lines were exposed to erlotinib (30 µM) for 4 h, the ER10, ER20, and ER30 samples without erlotinib were grown 24 h in the absence of erlotinib, while ER10, ER20, and ER30 samples with erlotinib were grown in media containing 10, 20, and 30 µM erlotinib, respectively, before being harvested for the western blotting in (c).