Direct evidence for dynamics of cell heterogeneity in watercored apples: turgor-associated metabolic modifications and within-fruit water potential gradient unveiled by single-cell analyses

摘要

Total soluble solids (TSS) (a), cell turgor (b), osmotic potential determined by vapor pressure method (c), vapor pressure method-based calculated water potential (d), osmotic potential determined by freezing point method (e) and vapor pressure method-based calculated water potential (f) in each tissue in the watercored apple fruit. The osmotic potential determined by the vapor pressure method was plotted against the osmotic potential determined by the freezing point method in each tissue (g). The osmotic potential differences in each position were shown in h. The data in a, c–f and h indicate that the means ± SE of 14–15 tissues collected from 10 fruit in each treatment. The data in b indicate the means ± SE of 40–47 cells collected from 10 fruit in each treatment. Different letters indicate a significant difference (Tukey–Kramer test, p < 0.05). For watercore, asterisks are shown on the bracket across c–e and d–f indicate significance at the 0.05 probability level by t-test. The dashed line in g indicates a 1:1 line. Bars = 5 cm