Cellular Localization of Two Rickettsia Symbionts in the Digestive System and within the Ovaries of the Mirid Bug Macrolophous pygmaeus

摘要

Like most insects, those that feed on both prey and plant materials harbor symbiotic bacteria in their body. Yet the involvement of bacteria in the feeding habits of these omnivorous consumers has yet to be investigated. In the present study, we took the first step toward testing the hypothesis that bacterial symbionts are involved in the feeding habits of the omnivorous bug Macrolophus pygmaeus. We (I) characterized the microbiome (the assembly of bacteria and fungi) of M. pygmaeus, and (II) determined the identity and location of the most dominant bacteria species within the host body. We found that M. pygmaeus microbiome is dominated by two Rickettsia species, R. belli and R. limoniae. These bacteria are found in high numbers in the digestive system of the bug, each exhibiting a unique distribution pattern, and for the most part, do not share the same cells in the gut. These results strongly suggest that the host bug may gain some nutritional benefits by hosting the two dominant symbiotic bacteria in its gut.