Characterization of Sodium Channel Mutations in the Dengue Vector Mosquitoes Aedesaegypti and Aedesalbopictus within the Context of Ongoing Wolbachia Releases in Kuala Lumpur Malaysia

摘要

Mosquitoes, Aedes aegypti and Ae. albopictus are vectors of dengue and must be controlled to prevent and contain outbreaks of this disease. Control by insecticide application is common and pyrethroid insecticides provide rapid knockdown of mosquitoes combined with relatively low mammalian toxicity. However, resistance to pyrethroids and other chemicals is causing problems for mosquito control around the world. In Malaysia, an alternative method of dengue reduction is employed which comprises releases of Ae. aegypti mosquitoes infected with a bacterium, Wolbachia, found naturally in other insects. Wolbachia turns the mosquitoes into incompetent vectors so they do not transmit the disease. Wolbachia mosquitoes are reared in the laboratory before release and must be able to survive in the field where they will encounter insecticides. Our study demonstrates benefits of crossing laboratory mosquitoes to those from the field over generations, so that the mosquito lines acquire field resistance characteristics (mutations in the sodium channel gene). We demonstrate that resistance mutations provide a survival advantage to Wolbachia Ae. aegypti mosquitoes, which must be maintained in laboratory lines by regular backcrossing. We also describe appearance of a sodium channel mutation in Malaysian Ae. albopictus which may indicate that pyrethroid resistance is increasing in this species.