Galectin-9 is required for endometrial regenerative cells to induce long-term cardiac allograft survival in mice

摘要

ERC characteristics and Gal-9 expression evaluation. A FACS analysis of ERC surface markers (CD29, CD45, CD90, and CD105). B Morphology of p2-p5 passage ERCs. C Gal-9 expression in p2-p5 ERCs were measured by ELISA, and there was no statistical difference among different generations (n = 3). D Gal-9 expression in ERCs was elevated by IFN-γ stimulation in a dose-dependent manner (n = 3). E Surface expression of Gal-9 in ERCs and IFN-γ-pre-stimulated ERCs (20 ng/ml, 72 h) were detected by flow cytometry. F Relative mRNA expression difference was analyzed between ERCs and IFN-γ-pre-stimulated ERCs (20 ng/ml, 72 h) by RT-PCR (n = 3). G Gal-9 protein expression differences in ERCs and IFN-γ-pre-stimulated ERCs (20 ng/ml, 72 h). H Gray value analysis based on western blot; Gal-9 intensity analysis was homogenized after comparing with HSP-90. I Immunofluorescence staining was performed to verify Gal-9 expression among ERCs and IFN-γ-pre-stimulated ERCs (20 ng/ml, 72 h). Gal-9 (green) and nuclei (blue) were merged together in (c) and (f). Differences among groups were assessed by using one-way analysis of variance (ANOVA) (groups ≧ 3) or unpaired t test (groups = 2).*p < 0.05, **p < 0.01. Abbreviations: ERC, endometrial regenerative cell; Gal-9, Galectin; p2-p5, passage 2-passage 5; wb, western blot; HSP-90, Heat shock protein 90