Combined therapy with adipose tissue-derived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis

摘要

In vitro infection of C57BL/6-derived peritoneal macrophages in co-culture with bone marrow (BM)-derived mesenchymal stromal cells (MSCs) or adipose tissue (AD)-derived MSCs. Representative images of peritoneal macrophages after infection with Leishmania amazonensis and macrophages and amastigote counts under the microscope. a Culture of infected macrophages. b Co-culture of infected macrophages with BM-MSCs. c Co-culture of infected macrophages with AD-MSCs. d Total amastigotes per macrophage. e Total amastigotes per well. f NO measurement. Rapid panoptic stain. Scale 20 μm. (N = 2) Representative of two independent experiments. Values show the mean ± standard deviation. *Significantly different from infected Mf without co-culture (control) (P < 0.05). #Significantly different from infected Mf treated with BM-MSC (P < 0.05)