Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion

摘要

Schematic workflow for lung burden analysis. a, Selection of the optimal wavelength to quantify CNM concentration without interference from the bio-components of the tissue homogenate. b Quantification of CNM concentration after mixing with lung tissue homogenates. In this step, proteinase K (PK) digestion was used to collect CNMs from lung tissue homogenates. c, in vivo evaluation of this lung burden assay using a murine pharyngeal aspiration model