Engineering transcription factor-based biosensors for repressive regulation through transcriptional deactivation design in Saccharomyces cerevisiae

摘要

Design of the repressive biosensor through transcriptional deactivation in response to an increase in ligand. The transcription factor (TF) was fused to the activation domain (AD) of the transcriptional activator. Contrary to the biosensor for transcriptional activation which operator was inserted into the core promoter close to the TATA box, the operators of the TF were inserted into the upstream region of the promoter to design the biosensor for transcriptional repression. When the ligand concentration was low, TF-AD binded to the operator and recruited the transcription machinery to activate the expression of an output signal (GFP). When the ligand concentration increased, TF-AD released from the operator and deactivated the expression of GFP