PSVI-10 Essential oils and benzoic acid associated or not with antibiotic in diets for weaned piglets on cecum microbiota composition

摘要

This study evaluated the effects of an alternative feed additive (benzoic acid and the essential oils of eugenol, thymus and piperine), associated or not with colistin, in weaned piglets feeding on α and β bacterial diversity, and on the percentages of operational taxonomic unit of Lactobacillus, Bifidobacterium, Clostridium, Escherichia, and Campylobacter in the cecum content of pigs. One hundred and eight crossbred piglets (5.3 ± 0.5kg) were housed 3 per pen and fed the following diets: a control diet (CON) with no addition of growth promoter feed additive; CON + 40 ppm of colistin; CON + 0.3% of essential oils and benzoic acid; and CON + 0.3% of essential oils and benzoic acid, and 40 ppm of colistin. The diets were based on corn, soybean meal, dairy products, and spray-dried blood plasma, and were formulated to provide 3.40 Mcal of ME/kg; 220 g of CP/kg; and 14.5 g of digestible lysine/kg. The animals were sanitary challenged, by spraying 2 L of diluted pig manure (60 % of manure to 40 % of water) at each nursery pen two days before the beginning of the trial. At the 14th day of the trial, 9 animals per diet were euthanized, and the cecum content was collected for microbial DNA extraction and sequencing. The data was submitted to ANOVA using the PROC MIXED of SAS. The α and β bacterial diversity, and the percentages of Lactobacillus, Bifidobacterium and Clostridium in the cecum of pigs were not affected (P > 0.05) by the experimental diets. There was a tendency (P = 0.09) for a lower concentration of Escherichia (3.5 vs. 1.46%) and lower percentage of Campylobacter (10.21 vs. 0.52%) in the cecum content of piglets fed diets containing essential oils and benzoic acid compared to pigs fed diets without the alternative feed additive. Inclusion of benzoic acid and essential oils in piglets feeding was effective in controlling intestinal pathogenic bacteria.