Upregulation of the miRNA-155 miRNA-210 and miRNA-20b in psoriasispatients and their relation to IL-17

摘要

Psoriasis is an immune-mediated disease, with genetic background and triggeringenvironmental factors; however, several gaps are still present in understandingthe intertwined relationship between these elements. Epigenetic mechanisms,including microRNAs (miRNAs), play an important role in the pathogenesis ofpsoriasis. The relationship between interleukin (IL)-17, a key cytokine inpsoriasis, and these epigenetic mechanisms still needs to be elucidated. Thisstudy aimed at assessing the expression of miRNA-155, miRNA-210, and miRNA-20bin skin and sera of psoriasis patients in relation to IL-17 levels. For 20psoriasis patients and 20 matching controls, the expression of miRNA-155,miRNA-210, and miRNA-20b was assessed using real-time polymerase chain reaction(RT-PCR), whereas IL-17/IL-17A levels were measured using quantitativeenzyme-linked immunosorbent assay (ELISA) technique. MiRNA-155 expression wassignificantly higher in lesional skin compared to controls(P = 0.001). MiRNA-210 expression was significantly higher inboth, lesional skin (P = 0.010) and sera of patients(P = 0.001) in comparison with controls. A statisticallysignificant positive correlation was found between serum miRNA-210 expressionand serum levels of IL-17/IL-17A (P = 0.010, rs = 0.562).MiRNA-20b lesional and non-lesional expression was significantly higher thancontrols (P < 0.001; P = 0.018). Inconclusion, the expression of miRNA-155, miRNA-210, and miRNA-20b is exaggeratedin psoriasis and they may be involved in disease pathogenesis. A possiblerelationship between miRNA-210 and IL-17 may be suggested; however, furtherstudies are still needed to verify this relation.