a Single-guide RNA sequences were designed against exon 8 of the NF2 gene (left side) and exon 1 of the CDKN2A (p16) gene (right side). b Independent NF2-knockout clones (NF2-KO#1 and #2), p16-knockout clones (p16-KO#1 and #2), and NF2/p16-DKO clones (DKO#1 and #2) were established. NF2 and p16/14 protein expression was determined by western blot analysis. GAPDH was used as an internal standard.
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