Involvement of CDKN1A (p21) in cellular senescence in response to heat and irradiation stress during preimplantation development

摘要

a qRT-PCR analysis of Cdkn1a transcripts in preimplantation embryos. Expression data from each stage were normalized to an internal control (Chuk) and are relative to one-cell zygotes. b Expression and subcellular localization of CDKN1A protein at morula. Error bars indicate the mean ± SEM. RQ, relative quantification. Different letters indicate significant differences (P < 0.05). Heat stress (exposed to 39 °C.); irradiation (exposed to 1-Gy X-ray)