Development of a computer-based quantification method for immunohistochemically-stained tissues and its application to study mast cells in equine wound healing (proof of concept)

摘要

Study design. Full-thickness excisional wounds were created on the thorax (15 cm2) and the forelimb (6 cm2) (2 per anatomical site) on 4 mixed breed mares and left to heal by second intention. Samples were harvested alternatively between the two wounds/site on days 1, 3, 8 and 17. Based on inclusion and exclusion criteria, a total of 26 healthy wounds were included. Two specimens showed clinical signs of EGT and 3 others received NPWT treatment, all harvested from limb wounds, and were thus excluded. The photomicrograph was taken at a 400x magnification. NPWT: negative pressure wound therapy, EGT: exuberant granulation tissue