Two Nucleoporin98 homologous genes jointly participate in the regulation of starch degradation to repress senescence in Arabidopsis

摘要

Mutation of Nup98 leads to an early senescence phenotype. a: T-DNA insertion alleles in Nup98a and Nup98b. Black bars indicate exons, thin black lines indicate introns or UTR, open lines in the 5′-UTR indicates an intron, triangles show T-DNA insertions with the mutant name and T-DNA identifier above the symbol. b: RT-PCR confirmed the single mutants of nup98a-1, nup98a-2, and nup98b-1. The position of forward (F) and reverse (R) primers are indicated in a (black arrows). ACT2 was used as a control. b refers to the full-length image being available in Figure SI. c: Single mutants of nup98a-1, nup98a-2, and nup98b-1 displayed similar phenotypes to wild type plants. However the double mutants of nup98a-1 nup98b-1 and nup98a-2, nup98b-1 showed severe early senescence. The photographs were taken on day 20 after germination. d: The leaf senescence phenotype of the nup98a-1 nup98b-1 double mutant rosette leaves harvested at inflorescence emergence. e: Ectopic expression of Nup98b rescued the leaf senescence phenotype of the nup98a-1 nup98b-1 double mutant. All plants were grown in soil from germination. The photographs were taken on day 30 after germination. An * indicates measurements that were significantly (*P < 0.05 or **P < 0.01) different from the control. Error bars indicate ± SD of the mean. All the images are our own data