Multiple mitochondrial pathways were activated by mitochondrial-damaging agents. a, c, e Fluorescent images and b, d, f quantification of GFP fluorescence of C. elegans carrying a, b3XESRE::GFP; c, dPhsp-6::GFP; or e, fPtbb-6::GFP reporters that were treated for 10 h with 50 μM rotenone, 3 mM TTFA, 50 μM antimycin A, 10 mM sodium azide, 20 μM CCCP, or vehicle (DMSO). g Fluorescent images of worms with 3XESRE::GFP (most left), Phsp-6::GFP (second from the left), Ptbb-6::GFP (second from the right), or Phsp-16.1::GFP (most right) reared on E. coli containing empty vector (EV) (top) or spg-7(RNAi) (bottom). Representative images are shown; three biological replicates with ~ 400 worms/replicate were analyzed. Error bars represent SEM. p values were determined from one-way ANOVA, followed by Dunnett’s test. All fold changes were normalized to DMSO control. NS not significant, *p < 0.05, **p < 0.01, ***p < 0.001. See Fig. S1 in Additional File 1 for quantification for g
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机译：通过线粒体损伤剂激活多种线粒体途径。 A，C，E荧光图像和B，D，F量化GFP荧光的C.秀丽隐叶携带，B3xesre :: GFP; C，DPHSP-6 :: GFP;或者，FPTBB-6 :: GFP记者用50μm旋转酮，3mM TTFA，50μM抗霉素A，10mM叠氮化物，20μMCCCP或载体（DMSO）处理。 g荧光图像的蠕虫与3xesre :: gfp（最左），phsp-6 :: gfp（左边的第二个），ptbb-6 :: gfp（从右边的第二个），或phsp-16.1 :: gfp（大多数右侧）饲养含有空载体（EV）（顶部）或SPG-7（RNAi）（底部）的大肠杆菌上。示出了代表性图像;分析了〜400蠕虫/复制的三种生物重复。误差栏代表SEM。从单向ANOVA确定P值，然后是Dunnett的测试。所有折叠变化都被标准化为DMSO控制。 ns不显着，* p <0.05，** p <0.01，*** p <0.001。见图。S1在附加文件1中，用于G的量化
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