A chromosome-level assembly of the cat flea genome uncovers rampant gene duplication and genome size plasticity

摘要

C. felis genome characteristics. a Summary statistics for long-read sequencing, assembly, and gene annotation. b Of 16,518 total protein-encoding genes (BIG9 scaffolds), 10,088 are derived from gene duplications (6225 duplication events within 3863 OGs at a threshold of 90% aa identity). c Assessment of the number of genes per duplication (left) and the relative distances between duplicate genes (right). Distances were computed only for true duplications (n = 2 genes) at a threshold of 90% aa identity. d Gene duplications are enriched within BIG9 scaffolds (tandem and proximal, red numbers) versus across scaffolds (dispersed, black numbers). e Enriched cellular functions of duplicate genes relative to single-copy genes. fC. felis belongs to a minimal fraction of eukaryotes containing abundant tRNA genes. tRNA gene counts are shown for disease vectors (VectorBase [26]) and eukaryotes carrying over 1000 tRNA genes (GtRNAdb [27]); ratios show the number of genomes with > 1000 tRNA genes per taxon